400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
T7 Tag Peptide 是一个蛋白标签，由 T7 噬菌体衣壳蛋白 gp 10 N-端的 11个残基组成。T7 Tag Peptide 可用于不同的免疫检测以及亲和纯化。
编号：173612
CAS号：245445-88-9
单字母：H2N-MASMTGGQQMG-OH
T7 Tag源自T7噬菌体衣壳蛋白的表位标签。
T7 Tag Peptide 是一个蛋白标签，由 T7 噬菌体衣壳蛋白 gp 10 N-端的 11个残基组成。T7 Tag Peptide 可用于不同的免疫检测以及亲和纯化。
T7 Tag Peptide is a protein tag derived from the N-terminal 11 residues of the major T7 capsid protein, gp 10. T7 Tag Peptide can be used in different immunoassays as well as affinity purification.
T7标签是一种表位标签，由T7噬菌体基因10的前导序列编码的11肽组成。该基因编码T7主要衣壳蛋白，其功能还不清楚。表位标签可用于标记和检测蛋白，适用于免疫印迹、免疫沉淀和免疫染色技术。由于其体积小，它们不太可能影响标记蛋白的生化特性。
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
T7标签通常与靶蛋白的N端或C端连接，使标记蛋白可用免疫化学的方法进行分析和可视化。T7标签作为一般的表位标签被广泛用于许多表达载体，包括基于T7 RNA聚合酶表达系统的pET系统。
The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems [1].
References:
1. Studier, F.W. and Moffatt, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-30.
专肽生物可以提供常见抗体制备、免疫学研究等生物科研需求的各类标签多肽（Tag peptide）以及蛋白底物产品，可用荧光标记(FITC、5-Fam)等。除常见抗体中抗原决定部位的标签多肽（tag: His | c-Myc | HA | FLAG | V5 | X-press | VSV tag | T7 tag | Snoop tag | E-tag | Sof tag 1 | Spy tag |等等），我们还提供各类定制标签肽的合成，并可提供荧光标记(FITC、5-Fam)等。同时，免费提供的MS和HPLC分析报告。
| DOI | 名称 | |
|---|---|---|
| 10.1016/0022-2836(86)90385-2 | Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes | 下载 |
| 10.11491/apcche.2004.0.377.0 | Asian Pacific Confederation of Chemical Engineers congress program and abstracts | 下载 |
| 编号 | 名称 | CAS号 |
| 407315 | 多肽标签Cys-T7 tag | |
| H2N-CMASMTGGQQMG-OH | ||
| T7 Tag Peptide的N端引入Cys氨基酸 | ||
| 407316 | 多肽标签T7 tag-Cys | |
| H2N-MASMTGGQQMGC-OH | ||
| T7 Tag Peptide的C端引入Cys氨基酸 | ||
