400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
V5表位标签肽三氟乙酸盐是一种衍生自猿猴病毒5副粘病毒P和V蛋白上存在的小表位的标签肽。
编号：135352
CAS号：
单字母：H2N-GKPIPNPLLGLDST-OH
V5是从猿病毒5(SV5)副粘病毒P蛋白和V蛋白中分离得到的14个氨基酸的多肽。
V5 Epitope Tag Peptide Trifluoroacetate 是一种多肽，源于猴病毒5的副粘病毒的 P 和 V 蛋白上的抗原决定簇。
V5 Epitope Tag Peptide Trifluoroacetate is a tag peptide derived from a small epitope present on the P and V proteins of the paramyxovirus of simian virus 5.
被识别的V5抗原表位是simian parainfluenza virus type 5（Southernet al., 1991）的RNA聚合酶α亚基中的序列95GKPIPNPLLGLDST108，由于在许多不同物种中都可以产生高亲和性的抗体，因而选择该短肽序列设计标签肽[1]。
含有14个氨基酸的V5表位被插入衣壳蛋白的C端（在ORF2终止密码子之前），从而构建一个重组标记病毒。V5 epitope被展示在衣壳蛋白的表面。在体外和小鼠中，重组标记病毒与亲本病毒具有相似的活动，可以通过聚合酶链反应（PCR）和血清学的方法加以区分[2，3]。
V5/His-epitope tag加入到每个编码序列的氨基末端用以区分质粒编码的蛋白和内源性LRRK2。质粒瞬时转染COS7细胞后，细胞裂解物用V5 epitope tag或LRRK24特异性的抗体通过SDS-PAGE和Western blotting的方法进行分析。
The recognized V5 epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase α subunit of simian parainfluenza virus type 5. This short peptide sequence was chosen because high-affinity antibodies can be reliably produced in many different species1.
A 14-amino-acid V5 epitope derived from simian parainfluenza virus type 5 (Southernet al., 1991) was inserted into the C terminus of the capsid protein (before the stop codon ofORF2) to construct a recombinant marker virus. We demonstrated that the V5 epitope was displayed on the surface ofthe capsid protein. Furthermore, the recombinant marker virus behaved similarly to the parental virus in vitro and in mice, and could be differentiated from the parental virus via polymerase chain reaction (PCR) and serological methods2, 3.
A V5/His-epitope tag was added to the amino terminus of each coding sequence to distinguish the plasmid-encoded protein from endogenous LRRK2. Plasmids were transiently transfected into COS7 cells, and cell lysates were analyzed by SDS-PAGE and Western blotting with antibodies specific for the V5 epitope tag or LRRK24.
References:
1. Zhou,W.H.,Dong, L.L., Ginsburg,D.,Bouhassira, E.E., Tsai,H.M.,2005. Enzymatically active ADAMTS 13 variants are not inhibited by anti-ADAMTS 13 autoantibodies: a novel therapeutic strategy? J. Biol. Chem. 280, 39934–39941.
2. Southern,J.A.,Young,D.F.,Heaney, F.,Baumgartner,W.K.,Randall,R.E.,1991.Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72,1551–1557
3. L. Huang, Y. Lu et al. Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research. 161 (2011) 115–123
4. D. MacLeod,J. Dowman, The Familial Parkinsonism Gene LRRK2Regulates Neurite Process Morphology. Neuron 52, 587–593, 2006
专肽生物可以提供常见抗体制备、免疫学研究等生物科研需求的各类标签多肽（Tag peptide）以及蛋白底物产品，可用荧光标记(FITC、5-Fam)等。除常见抗体中抗原决定部位的标签多肽（tag: His | c-Myc | HA | FLAG | V5 | X-press | VSV tag | T7 tag | Snoop tag | E-tag | Sof tag 1 | Spy tag |等等），我们还提供各类定制标签肽的合成，并可提供荧光标记(FITC、5-Fam)等。同时，免费提供的MS和HPLC分析报告。
| DOI | 名称 | |
|---|---|---|
| 10.1074/jbc.M104489200 | Identification of peptide ligand-binding domains within the human motilin receptor using photoaffinity labeling | 下载 |
| 10.1152/ajpgi.00305.2004 | Signaling pathways mediating gastrointestinal smooth muscle contraction and MLC20 phosphorylation by motilin receptors | 下载 |
| 10.1111/jth.13688 | Enzymatically active ADAMTS 13 variants are not inhibited by anti-ADAMTS 13 autoantibodies: a novel therapeutic strategy? | 下载 |
| 10.1099/0022-1317-72-7-1551 | Identi?cation of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. | 下载 |
| 10.1016/j.virusres.2011.05.015 | Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research. | 下载 |
| 10.1016/j.neuron.2006.10.008 | The familial Parkinsonism gene LRRK2 regulates neurite process morphology | 下载 |
