400-998-5282
专注多肽 服务科研
400-998-5282
专注多肽 服务科研
PACAP (1-38), human, ovine, rat 是一种含有 38 个氨基酸残基的神经肽。
编号：137743
CAS号：137061-48-4
单字母：H2N-HSDGIFTDSYSRYRKQMAVKKYLAAVLGKRYKQRVKNK-CONH2
PACAP (1-38), human, ovine, rat TFA 是一种含有 38 个氨基酸残基的神经肽。PACAP (1-38) 结合到 PACAP I 型受体，PACAP II 型受体 VIP1 和 PACAP II 型受体 VIP2，IC50 分别为 4 nM，2 nM 和 1 nM。
PACAP (1-38), human, ovine, rat TFA is a neuropeptide with 38 amino acid residues. PACAP (1-38) binds to PACAP type I receptor, PACAP type II receptor VIP1, and PACAP type II receptor VIP2 with IC50s of 4 nM, 2 nM, and 1 nM, respectively.
PACAP (1-38) 是一种有效的PACAP受体激动剂（Kd=100 pM），它能够刺激腺苷酸环化酶和吞噬作用。PACAP (1-38) 能够抑制p38 MAPK及PAC1和VPAC1介导的NF-κB的易位而导致的肾脏近曲小管上皮细胞损伤。PACAP (1-38) 抑制骨髓瘤细胞的生长，可能是通过间接地抑制骨髓基质细胞中生长因子IL-6的产生。体内研究中，PACAP (1-38)能够有效地抑制轻链诱导的细胞因子的表达并阻止所造成的细胞损伤。
PACAP (1-38), human, ovine, rat 是一种含有 38 个氨基酸残基的神经肽。PACAP (1-38) 结合到 PACAP I 型受体，PACAP II 型受体 VIP1 和 PACAP II 型受体 VIP2，IC50 分别为 4 nM，2 nM 和 1 nM。
垂体腺苷酸环化酶激活多肽（PACAP）是一种多效性神经肽，在中枢神经系统及外周组织中具有广泛生物活性。该多肽通过激活PAC1和VPAC1受体，既能发挥神经保护作用，又具有抗炎效应——这两种机制均与神经退行性疾病的发病及进展密切相关。
Design and in vitro characterization of PAC1/VPAC1-selective agonists with potent neuroprotective effects 阐明了PACAP氨基末端的分子结构特征，并开发能促进神经元存活的强效选择性PAC1/VPAC1受体激动剂。并合成了一系列PACAP衍生物，分别检测了它们对PAC1、VPAC1和VPAC2受体的结合亲和力及诱导细胞内钙动员的能力。最终，在神经元损伤体外模型中评估了具有优化药理学特性的类似物。
研究结果显示：在第2位引入羟脯氨酸或第7位引入丙氨酸的衍生物均未表现出显著VPAC2受体激动活性。构效关系研究表明，可能存在共同（Asx-turn样）和独特（不同N端封盖类型）的二级结构，这些结构可能决定受体的识别、选择性和激活。值得注意的是，在预处理和共处理实验中，[Ala7]PACAP27和[Hyp2]PACAP27均展现出显著保护人多巴胺能SH-SY5Y神经母细胞免受MPP+毒性的能力。这些新的药理学和结构数据将为合理设计PACAP衍生化合物提供重要依据，这类化合物有望成为治疗神经退行性疾病的潜在治疗药物。
体外研究
PACAP (1-38)，human，ovine，rat 是垂体腺苷酸环化酶激活多肽的片段。
PACAP (1-38) 对来自各种组织 (包括内分泌胰腺) 的膜中的 PACAP 特异性 (PAC1) 受体表现出高亲和力。
在体外，PACAP (1-38) 松弛由组胺和乙酰胆碱预收缩的豚鼠和兔气管平滑肌。PACAP (1-38) 还增加气管平滑肌中的腺苷 3':5'-环磷酸 (环 AMP)，为 PACAP (1-38) 的松弛作用提供了可能的机制。
体内研究
在假动物中单独使用 PACAP (1-38) 不会导致任何视网膜层发生变化。PACAP (1-38) 溶解在 solutio ophthalmica cum benzalkonio 中，可显著保护双侧颈总动脉闭塞 (BCCAO) 受损视网膜的视网膜；与对照视网膜相比，用 PACAP (1-38) 滴眼液处理的视网膜结构得以保留。与假手术对照组相比，BCCAO 视网膜中的 OLM-ILM (外界膜-内界膜) 距离减少了 49.7% (p<0.001)，但在接受 PACAP 处理的眼睛中仅为 40.6% (p<0.001) (1-38) 眼药水。在内核层 (INL) (BCCAO：38.5%，PACAP (1-38)：30.5%；p<0.001) 和内丛状层 (IPL) (BCCAO：64.8%，PACAP (1-38)：38.2%；p<0.05)，而在外核层 (ONL) 中未观察到具有统计学意义的损伤衰减 (BCCAO：36.5%，PACAP (1-38)：37.7%) 或外丛状层 (OPL) (BCCAO：53.0%，PACAP (1-38)：48.2%)。BCCAO 后神经节细胞层 (GCL) 细胞数量显著减少 52.4% (p<0.05)，PACAP (1-38) 滴眼液显著改善 (减少 25.9%；p<0.05)。
参考文献
[1]. Gourlet P, et al. Fragments of pituitary adenylate cyclase activating polypeptide discriminate between type I and II recombinant receptors. Eur J Pharmacol. 1995 Dec 4;287(1):7-11.
[2]. Yamaguchi N. Pituitary adenylate cyclase activating polypeptide enhances glucose-evoked insulin secretion in the canine pancreas in vivo. JOP. 2001 Sep;2(5):306-16.
[3]. Lindén A, et al. Inhibition of bronchoconstriction by pituitary adenylate cyclase activating polypeptide (PACAP 1-27) in guinea-pigs in vivo. Br J Pharmacol. 1995 Jul;115(6):913-6.
[4]. Werling D, et al. Passage through the Ocular Barriers and Beneficial Effects in Retinal Ischemia of Topical Application of PACAP (1-38) in Rodents. Int J Mol Sci. 2017 Mar 21;18(3). pii: E675.
| DOI | 名称 | |
|---|---|---|
| 10.1016/0014-2999(95)00467-5 | Fragments of pituitary adenylate cyclase activating polypeptide discriminate between type I and II recombinant receptors | 下载 |
| 10.3390/ijms18030675 | Passage through the Ocular Barriers and Beneficial Effects in Retinal Ischemia of Topical Application of PACAP1-38 in Rodents | 下载 |
多肽H2N-His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取1.13g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取1.02mmol Fmoc-Lys(Boc)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入2.03mmol DIPEA，0.97mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Lys(Boc)-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Lys(Boc)-Linker-MBHA Resin
Fmoc-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Asp(OtBu)-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Asp(OtBu)-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
H2N-Ser(tBu)-Asp(OtBu)-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
Fmoc-His(Trt)-Ser(tBu)-Asp(OtBu)-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-His(Trt)-Ser(tBu)-Asp(OtBu)-Gly-Ile-Phe-Thr(tBu)-Asp(OtBu)-Ser(tBu)-Tyr(tBu)-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Arg(Pbf)-Lys(Boc)-Gln(Trt)-Met-Ala-Val-Lys(Boc)-Lys(Boc)-Tyr(tBu)-Leu-Ala-Ala-Val-Leu-Gly-Lys(Boc)-Arg(Pbf)-Tyr(tBu)-Lys(Boc)-Gln(Trt)-Arg(Pbf)-Val-Lys(Boc)-Asn(Trt)-Lys(Boc)-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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